Mouse Liver Organoid

Description

Mouse liver organoids are derived from embryonic or neonatal mouse liver tissue, where hepatic progenitor cells (HPCs), liver mesenchymal cells (LMCs), and liver sinusoidal endothelial cells (LSECs) can be isolated in parallel, minimizing donor-to-donor variability while preserving stemness and physiological relevance. Organoids typically appear as cystic or solid spheroids and branching cholangiocyte-like structures, forming bile canaliculi–like networks; Kupffer cells and hepatic stellate cells can be incorporated to model immune and fibrotic responses. With small body size, short breeding cycles, and easy husbandry, mouse liver organoids are widely used for liver disease modeling (metabolism, inflammation, fibrosis), drug screening and DILI/toxicology, ADME/DMPK studies, and liver–immune crosstalk. All organoids are screened for mycoplasma, bacteria, and fungi/yeast, and are verified for identity, genetic stability, and functional performance (e.g., albumin secretion, urea production, CYP450 induction)